Analysis Note

Positive ControlHepG2 cells

Negative ControlHS27 cells

Application

Immunoblotting (not recommended)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1-2 µg/sample)r>Neutralization Studies (1 µg/ml; use Cat. No. GR11L)r>Paraffin Sections (not recommended)

General description

Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of IGF-IR (Ab-1).

Purified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

Immunogen

partially purified, human placenta IGF-I receptor

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Roth, R. 1988. Science239, 1269.Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.Rosen, O.M., 1987. Science257, 1452.Rechler, M.M. and Nissley, S.P., 1986. Hormone Res.24, 152.Ullrich, A., et al. 1986. EMBO J.5, 2503.Zapf, S. and Froesch, E.R., 1986. Hormone Res.24, 121.Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).Kull, F.C., et al. 1983. J. Biol. Chem.258, 6561.

Anti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)